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A unifying mathematical framework for experimental TCR-pMHC kinetic constants
dc.contributor.author | Faro Rivas, José Manuel | es-ES |
dc.contributor.author | Castro Ponce, Mario | es-ES |
dc.contributor.author | Molina París, Carmen | es-ES |
dc.date.accessioned | 2017-12-21T15:45:28Z | |
dc.date.available | 2017-12-21T15:45:28Z | |
dc.date.issued | 2017-04-26 | es_ES |
dc.identifier.issn | 2045-2322 | es_ES |
dc.identifier.uri | https:doi.org10.1038srep46741 | es_ES |
dc.description | Artículos en revistas | es_ES |
dc.description.abstract | es-ES | |
dc.description.abstract | Receptor binding and triggering are central in Immunology as T cells activated through their T cell receptors (TCR) by protein antigens orchestrate immune responses. In order to understand receptor-ligand interactions, many groups working with different experimental techniques and assays have generated a vast body of knowledge during the last decades. However, in recent years a type of assays, referred to as two-dimensional or membrane-to-membrane, has questioned our current understanding of the role of different kinetic constants (for instance, on- versus off-rate constants) on TCR-ligand interaction and subsequent T cell activation. Here we present a general mathematical framework that provides a unifying umbrella to relate fundamental and effective (or experimentally determined) kinetic constants, as well as describe and compare state-of-the-art experimental methods. Our framework is able to predict the correlations between functional output, such as 1EC50, and effective kinetic constants for a range of different experimental assays (in two and three dimensions). Furthermore, our approach can be applied beyond Immunology, and serve as a «translation method» for the biochemical characterization of receptor-ligand interactions. | en-GB |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | en-GB | es_ES |
dc.source | Revista: Scientific Reports, Periodo: 1, Volumen: online, Número: , Página inicial: 46741-1, Página final: 46741-12 | es_ES |
dc.subject.other | Instituto de Investigación Tecnológica (IIT) | es_ES |
dc.title | A unifying mathematical framework for experimental TCR-pMHC kinetic constants | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.rights.holder | es_ES | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.keywords | es-ES | |
dc.keywords | Computational models; Kinetics; Lymphocyte activation | en-GB |
dc.identifier.doi | 10.1038/srep46741 | es_ES |
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