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Título : | Glutathione-Triggered catalytic response of Copper-Iron mixed oxide Nanoparticles. Leveraging tumor microenvironment conditions for chemodynamic therapy |
Autor : | Bonet Aleta, Javier Sancho Albero, Maria Calzada Funes, Javier Irusta, Silvia Martín Duque, Pilar Hueso, Jose L. Santamaría, Jesús |
Fecha de publicación : | 1-jul-2022 |
Resumen : | . Heterogeneous catalysis has emerged as a promising alternative for the development of new cancer therapies. In addition, regarding the tumor microenvironment as a reactor with very specific chemical features has provided a new perspective in the search for catalytic nanoarchitectures with specific action against chemical species playing a key role in tumor metabolism. One of these species is glutathione (GSH), whose depletion is the cornerstone of emerging strategies in oncology, since this metabolite plays a pivotal regulatory role as antioxidant agent, dampening the harmful effects of intracellular reactive oxidative species (ROS). Herein, we present copper-iron oxide spinel nanoparticles that exhibit a versatile and selective catalytic response to reduce GSH levels while generating ROS in a cascade reaction. We demonstrate a clear correlation between GSH depletion and apoptotic cell death in tumor cells in the presence of the copper-iron nanocatalyst. Furthermore, we also provide a novel analytical protocol, alternative to state-of-the-art commercial kits, to accurately monitoring the concentration of GSH intracellular levels in both tumor and healthy cells. We observe a selective action of the nanoparticles, with lower toxicity in healthy cell lines, whose intrinsic GSH levels are lower, and intense apoptosis in tumor cells accompanied by a fast reduction of GSH levels. |
Descripción : | Artículos en revistas |
URI : | https://doi.org/10.1016/j.jcis.2022.03.036 http://hdl.handle.net/11531/98969 |
ISSN : | 0021-9797 |
Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
Fichero | Tamaño | Formato | |
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202553016218629_Art3_JournalColloidInterfaces.pdf | 3,35 MB | Adobe PDF | Visualizar/Abrir |
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