• English
    • español
  • español 
    • English
    • español
  • Login
Ver ítem 
  •   DSpace Principal
  • 2.- Investigación
  • Artículos
  • Ver ítem
  •   DSpace Principal
  • 2.- Investigación
  • Artículos
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Method Development and Validation for Phenol and Nitrophenols in Tap Water by HPLC using a Monolithic Column

Thumbnail
Ver/
IIT-06-082A.pdf (3.212Mb)
Fecha
01/01/2006
Autor
Cledera Castro, Mª del Mar
Santos Montes, Ana María
Izquierdo Hornillos, Roberto
Estado
info:eu-repo/semantics/publishedVersion
Metadatos
Mostrar el registro completo del ítem
Mostrar METS del ítem
Ver registro en CKH

Refworks Export

Resumen
 
 
An isocratic HPLC method for the determination of phenol and nitrophenols (4-nitrophenol, 2-nitrophenol, 4,6-dinitro-o-cresol and 2,4-dinitrophenol) has been developed and validated using 2-chlorophenol as internal standard (IS) and a monolithic column in tap water samples. Prior to HPLC, the method requires solid-phase extraction (SPE) using polymeric Lichrolut EN cartridges. The method development involved the study of methanol and acetonitrile as organic modifiers, pH and flow-rate using a Chromolith RP-18e (150 mm × 4.6 mm I.D.) column. After comparing the performance of the separations obtained with both organic modifiers, the optimum separation of these compounds was achieved using 50 mM acetate buffer (pH 5.0)-acetonitrile (80:20, v/v) as mobile phase, 3 mL min-1 flow-rate and UV detection at maximum absorbance wavelength. Under these conditions, all analytes were separated (Rs > 2.0) in an analysis time of less than 3.5 min and the most important validation parameters were evaluated. The recoveries obtained in the accuracy test for all phenols studied were in the 90 112\% range using a preconcentration factor of 40, and the intraday and interday precisions [expressed as coefficient of variation (CV)] were smaller than 15\%. Finally, the proposed method was applied to wastewater samples from several industries.
 
URI
http://hdl.handle.net/11531/5322
Method Development and Validation for Phenol and Nitrophenols in Tap Water by HPLC using a Monolithic Column
Tipo de Actividad
Artículos en revistas
ISSN
1471-6577
Materias/ categorías / ODS
Instituto de Investigación Tecnológica (IIT)
Palabras Clave


Colecciones
  • Artículos

Repositorio de la Universidad Pontificia Comillas copyright © 2015  Desarrollado con DSpace Software
Contacto | Sugerencias
 

 

Búsqueda semántica (CKH Explorer)


Listar

Todo DSpaceComunidades & ColeccionesPor fecha de publicaciónAutoresTítulosMateriasPor DirectorPor tipoEsta colecciónPor fecha de publicaciónAutoresTítulosMateriasPor DirectorPor tipo

Mi cuenta

AccederRegistro

Repositorio de la Universidad Pontificia Comillas copyright © 2015  Desarrollado con DSpace Software
Contacto | Sugerencias